• Immunohistochemistry analysis of paraffin-embedded mouse brain using TNFSF12 Antibody (STJ27626) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using TNFSF12 Antibody (STJ27626) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using TNFSF12 Antibody (STJ27626) at dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TNFSF12 antibody (STJ27626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TNFSF12 antibody (42-123) (STJ27626)

SKU:
STJ27626

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TNFSF12 (42-123) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TNFSF12
Gene ID: 407977/8742
Uniprot ID: TNF12_HUMAN
Immunogen Region: 42-123
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-123 of human TNFSF12 (NP_003800.1).
Immunogen Sequence: VSLGSRASLSAQEPAQEELV AEEDQDPSELNPQTEESQDP APFLNRLVRPRRSAPKGRKT RARRAIAAHYEVHPRPGQDG AQ
Tissue Specificity Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing.
Function Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 12
Apo3 Ligand
Tnf-Related Weak Inducer Of Apoptosis
Tweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5676594
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Tumor Necrosis Factor Ligand Superfamily Member 12
Secreted Form: Secreted
Isoform Twe-Pril: Cell Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Tumor Necrosis Factor Ligand Superfamily Member 12 antibody
Anti-Apo3 Ligand antibody
Anti-Tnf-Related Weak Inducer Of Apoptosis antibody
Anti-Tweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form antibody
Anti-TNFSF12 antibody
Anti-APO3L antibody
Anti-DR3LG antibody
Anti-UNQ181 antibody
Anti-PRO207 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance