![Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178234/370532/STJ11105590_1__25608.1713136486.jpg?c=1 "Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.")
![Immunofluorescence analysis of MCF7 cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178234/370533/STJ11105590_2__63434.1713136486.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from 293F cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178234/370534/STJ11105590_3__57132.1713136487.jpg?c=1 "Western blot analysis of lysates from 293F cells using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178234/370532/STJ11105590_1__25608.1713136486.jpg?c=1 "Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TJP1 |
| Gene ID: | 7082 |
| Uniprot ID: | ZO1_HUMAN |
| Immunogen Region: | 1500-1650 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1500-1650 of human ZO-1 (NP_003248.3). |
| Immunogen Sequence: | PQKSFPDKAPVNGTEQTQKT VTPAYNRFTPKPYTSSARPF ERKFESPKFNHNLLPSETAH KPDLSSKTPTSPKTLVKSHS LAQPPEFDSGVETFSIHAEK PKYQINNISTVPKAIPVSPS AVEEDEDEDGHTVVATARGI FNSNGGVLSSI |
| Tissue Specificity | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. |
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions. |
| Protein Name | Tight Junction Protein Zo-1 Tight Junction Protein 1 Zona Occludens Protein 1 Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650 Reactome: R-HSA-2028269 Reactome: R-HSA-351906 Reactome: R-HSA-8935964 Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Junction Tight Junction Gap Junction Cell Projection Podosome Moves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell Contact At Podosomal Sites Is Predominantly Localized In The Ring Structure Surrounding The Actin Core Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative Antibody Names | Anti-Tight Junction Protein Zo-1 antibody Anti-Tight Junction Protein 1 antibody Anti-Zona Occludens Protein 1 antibody Anti-Zonula Occludens Protein 1 antibody Anti-TJP1 antibody Anti-ZO1 antibody |
Information sourced from Uniprot.org