• Western blot analysis of various lysates using THEM4 Rabbit polyclonal antibody (STJ118385) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-THEM4 antibody (37-150) (STJ118385)

SKU:
STJ118385

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-THEM4 (37-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: THEM4
Gene ID: 117145
Uniprot ID: THEM4_HUMAN
Immunogen Region: 37-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-150 of human THEM4 (NP_444283.2).
Immunogen Sequence: SSEEVILKDCSVPNPSWNKD LRLLFDQFMKKCEDGSWKRL PSYKRTPTEWIQDFKTHFLD PKLMKEEQMSQAQLFTRSFD DGLGFEYVMFYNDIEKRMVC LFQGGPYLEGPPGF
Tissue Specificity Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation.
Post Translational Modifications Phosphorylated.
Function Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism. Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity. inhibits AKT1 phosphorylation and activity. enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane.
Protein Name Acyl-Coenzyme A Thioesterase Them4
Acyl-Coa Thioesterase Them4
Carboxyl-Terminal Modulator Protein
Thioesterase Superfamily Member 4
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-165158
Reactome: R-HSA-199418
Reactome: R-HSA-389357
Reactome: R-HSA-5218920
Reactome: R-HSA-77289
Cellular Localisation Cell Membrane
Cell Projection
Ruffle Membrane
Cytoplasm
Mitochondrion
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Released From The Mitochondria Into The Cytosol In Response To Apoptotic Stimuli
Alternative Antibody Names Anti-Acyl-Coenzyme A Thioesterase Them4 antibody
Anti-Acyl-Coa Thioesterase Them4 antibody
Anti-Carboxyl-Terminal Modulator Protein antibody
Anti-Thioesterase Superfamily Member 4 antibody
Anti-THEM4 antibody
Anti-CTMP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance