• Confocal imaging of paraffin-embedded Human colon cancer tissue using TFF1/pS2 Rabbit monoclonal antibody (STJ11103613, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from MCF7 cells, using TFF1/pS2 Rabbit monoclonal antibody (STJ11103613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TFF1/pS2 antibody (1-84) [S3MR] (STJ11103613)

SKU:
STJ11103613

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TFF1/pS2 (1-84) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFF1
Gene ID: 7031
Uniprot ID: TFF1_HUMAN
Immunogen Region: 1-84
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-84 of human TFF1/pS2 (NP_003216.1).
Immunogen Sequence: MATMENKVICALVLVSMLAL GTLAEAQTETCTVAPRERQN CGFPGVTPSQCANKGCCFDD TVRGVPWCFYPNTIDVPPEE ECEF
Tissue Specificity Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
Function Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
Protein Name Trefoil Factor 1
Breast Cancer Estrogen-Inducible Protein
Pnr-2
Polypeptide P1.A
Hp1.A
Protein Ps2
Database Links Reactome: R-HSA-9018519
Cellular Localisation Secreted
Alternative Antibody Names Anti-Trefoil Factor 1 antibody
Anti-Breast Cancer Estrogen-Inducible Protein antibody
Anti-Pnr-2 antibody
Anti-Polypeptide P1.A antibody
Anti-Hp1.A antibody
Anti-Protein Ps2 antibody
Anti-TFF1 antibody
Anti-BCEI antibody
Anti-PS2 antibody

Information sourced from Uniprot.org

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