• Immunofluorescence analysis of U2OS cells using TERF2IP antibody (STJ117357) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using TERF2IP Rabbit polyclonal antibody (STJ117357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TERF2IP antibody (1-280) (STJ117357)

SKU:
STJ117357

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TERF2IP (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TERF2IP
Gene ID: 54386
Uniprot ID: TE2IP_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TERF2IP (NP_061848.2).
Immunogen Sequence: MAEAMDLGKDPNGPTHSSTL FVRDDGSSMSFYVRPSPAKR RLSTLILHGGGTVCRVQEPG AVLLAQPGEALAEASGDFIS TQYILDCVERNERLELEAYR LGPASAADTGSEAKPGALAE GAAEPEPQRHAGRIAFTDAD DVAILTYVKENARSPSSVTG NALWKAMEKSSLTQHSWQSL KDRYLKHLRGQEHKYLLGDA PVSPSSQKLKRKAEEDPEAA DSGEPQNKRTPDLPEEEYV
Tissue Specificity Ubiquitous. Highly expressed.
Function Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
Protein Name Telomeric Repeat-Binding Factor 2-Interacting Protein 1
Terf2-Interacting Telomeric Protein 1
Trf2-Interacting Telomeric Protein 1
Dopamine Receptor-Interacting Protein 5
Repressor/Activator Protein 1 Homolog
Rap1 Homolog
Hrap1
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-171319
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174430
Reactome: R-HSA-174437
Reactome: R-HSA-2559586
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Telomere
Associates With Chromosomes
Both At Telomeres And In Extratelomeric Sites
Also Exists As A Cytoplasmic Form
Where It Associates With The Ikk Complex
Alternative Antibody Names Anti-Telomeric Repeat-Binding Factor 2-Interacting Protein 1 antibody
Anti-Terf2-Interacting Telomeric Protein 1 antibody
Anti-Trf2-Interacting Telomeric Protein 1 antibody
Anti-Dopamine Receptor-Interacting Protein 5 antibody
Anti-Repressor/Activator Protein 1 Homolog antibody
Anti-Rap1 Homolog antibody
Anti-Hrap1 antibody
Anti-TERF2IP antibody
Anti-DRIP5 antibody
Anti-RAP1 antibody
Anti-PP8000 antibody

Information sourced from Uniprot.org

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