• Western blot analysis of extracts of various cell lines, using TAS1R3 antibody (STJ112196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TAS1R3 antibody (400-570) (STJ112196)

SKU:
STJ112196

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAS1R3 (400-570) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAS1R3
Gene ID: 83756
Uniprot ID: TS1R3_HUMAN
Immunogen Region: 400-570
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-570 of human TAS1R3 (NP_689414.2).
Immunogen Sequence: VAQALHNTLQCNASGCPAQD PVKPWQLLENMYNLTFHVGG LPLRFDSSGNVDMEYDLKLW VWQGSVPRLHDVGRFNGSLR TERLKIRWHTSDNQKPVSRC SRQCQEGQVRRVKGFHSCCY DCVDCEAGSYRQNPDDIACT FCGQDEWSPERSTRCFRRRS RFLAWGEPAVL
Function Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Protein Name Taste Receptor Type 1 Member 3
Sweet Taste Receptor T1r3
Database Links Reactome: R-HSA-418594
Reactome: R-HSA-420499
Reactome: R-HSA-9717207
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Taste Receptor Type 1 Member 3 antibody
Anti-Sweet Taste Receptor T1r3 antibody
Anti-TAS1R3 antibody
Anti-T1R3 antibody
Anti-TR3 antibody

Information sourced from Uniprot.org

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