• Immunofluorescence analysis of U-2 OS cells using TDP-43/TARDB Rabbit polyclonal antibody (STJ119313) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TDP-43/TARDB in paraffin-embedded mouse brain using TDP-43/TARDB Rabbit polyclonal antibody (STJ119313) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TDP-43/TARDB in paraffin-embedded human gastric cancer using TDP-43/TARDB Rabbit polyclonal antibody (STJ119313) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TDP-43/TARDB in paraffin-embedded rat spleen using TDP-43/TARDB Rabbit polyclonal antibody (STJ119313) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TARDBP antibody (1-260) (STJ119313)

SKU:
STJ119313

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TARDBP (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TARDBP
Gene ID: 23435
Uniprot ID: TADBP_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TDP-43/TARDB (NP_031401.1).
Immunogen Sequence: MSEYIRVTEDENDEPIEIPS EDDGTVLLSTVTAQFPGACG LRYRNPVSQCMRGVRLVEGI LHAPDAGWGNLVYVVNYPKD NKRKMDETDASSAVKVKRAV QKTSDLIVLGLPWKTTEQDL KEYFSTFGEVLMVQVKKDLK TGHSKGFGFVRFTEYETQVK VMSQRHMIDGRWCDCKLPNS KQSQDEPLRSRKVFVGRCTE DMTEDELREFFSQYGDVMDV FIPKPFRAFAFVTFADDQI
Tissue Specificity Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
Post Translational Modifications Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress. Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Function RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner. Negatively regulates the expression of CDK6. Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner.
Protein Name Tar Dna-Binding Protein 43
Tdp-43
Cellular Localisation Nucleus
Cytoplasm
Stress Granule
Mitochondrion
Continuously Travels In And Out Of The Nucleus
Localizes To Stress Granules In Response To Oxidative Stress
A Small Subset Localizes In Mitochondria
Alternative Antibody Names Anti-Tar Dna-Binding Protein 43 antibody
Anti-Tdp-43 antibody
Anti-TARDBP antibody
Anti-TDP43 antibody

Information sourced from Uniprot.org

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