• Immunohistochemistry analysis of Transgelin (TAGLN) in paraffin-embedded human placenta using Transgelin (TAGLN) Rabbit polyclonal antibody (STJ28843) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Transgelin (TAGLN) in paraffin-embedded human colon using Transgelin (TAGLN) Rabbit polyclonal antibody (STJ28843) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse uterus, using Transgelin (TAGLN) Rabbit polyclonal antibody (STJ28843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from Rat uterus, using Transgelin (TAGLN) Rabbit polyclonal antibody (STJ28843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TAGLN antibody (1-201) (STJ28843)

SKU:
STJ28843

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAGLN (1-201) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAGLN
Gene ID: 6876
Uniprot ID: TAGL_HUMAN
Immunogen Region: 1-201
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human Transgelin (TAGLN) (NP_001001522.1).
Immunogen Sequence: MANKGPSYGMSREVQSKIEK KYDEELEERLVEWIIVQCGP DVGRPDRGRLGFQVWLKNGV ILSKLVNSLYPDGSKPVKVP ENPPSMVFKQMEQVAQFLKA AEDYGVIKTDMFQTVDLFEG KDMAAVQRTLMALGSLAVTK NDGHYRGDPNWFMKKAQEHK REFTESQLQEGKHVIGLQMG SNRGASQAGMTGYGRPRQII S
Function Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Protein Name Transgelin
22 Kda Actin-Binding Protein
Protein Ws3-10
Smooth Muscle Protein 22-Alpha
Sm22-Alpha
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Transgelin antibody
Anti-22 Kda Actin-Binding Protein antibody
Anti-Protein Ws3-10 antibody
Anti-Smooth Muscle Protein 22-Alpha antibody
Anti-Sm22-Alpha antibody
Anti-TAGLN antibody
Anti-SM22 antibody
Anti-WS3-10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance