• Western blot analysis of extracts of various cell lines, using (STJ11103658) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Syntaxin 16 in paraffin-embedded mouse testis tissue using Syntaxin 16 Rabbit monoclonal antibody (STJ11103658) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Syntaxin 16 rabbit monoclonal antibody (STJ11103658) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Syntaxin 16 in paraffin-embedded rat kidney tissue using Syntaxin 16 Rabbit monoclonal antibody (STJ11103658) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Syntaxin 16 rabbit monoclonal antibody (STJ11103658) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Syntaxin 16 in paraffin-embedded human small intestine tissue using Syntaxin 16 Rabbit monoclonal antibody (STJ11103658) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat brain using Syntaxin 16 rabbit monoclonal antibody (STJ11103658) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Syntaxin 16 in paraffin-embedded mouse kidney tissue using Syntaxin 16 Rabbit monoclonal antibody (STJ11103658) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using Syntaxin 16 Rabbit monoclonal antibody (STJ11103658) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Syntaxin 16 antibody (1-100) [S8MR] (STJ11103658)

SKU:
STJ11103658

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Syntaxin 16 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX16
Gene ID: 8675
Uniprot ID: STX16_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syntaxin 16 (O14662).
Immunogen Sequence: MATRRLTDAFLLLRNNSIQN RQLLAEQVSSHITSSPLHSR SIAAELDELADDRMALVSGI SLDPEAAIGVTKRPPPKWVD GVDEIQYDVGRIKQKMKELA
Tissue Specificity Ubiquitous.
Function SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
Protein Name Syntaxin-16
Syn16
Database Links Reactome: R-HSA-6811438
Reactome: R-HSA-6811440
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Iv Membrane Protein
Isoform C: Cytoplasm
Alternative Antibody Names Anti-Syntaxin-16 antibody
Anti-Syn16 antibody
Anti-STX16 antibody

Information sourced from Uniprot.org

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