• Western blot analysis of extracts of various cell lines, using SURF1 antibody (STJ114395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SURF1 antibody (80-275) (STJ114395)

SKU:
STJ114395

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SURF1 (80-275) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SURF1
Gene ID: 6834
Uniprot ID: SURF1_HUMAN
Immunogen Region: 80-275
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1).
Immunogen Sequence: QVQRRKWKLNLIAELESRVL AEPVPLPADPMELKNLEYRP VKVRGCFDHSKELYMMPRTM VDPVREAREGGLISSSTQSG AYVVTPFHCTDLGVTILVNR GFVPRKKVNPETRQKGQIEG EVDLIGMVRLTETRQPFVPE NNPERNHWHYRDLEAMARIT GAEPIFIDANFQSTVPGGPI GGQTRVTLRNEHLQYI
Function Component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly.
Protein Name Surfeit Locus Protein 1
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Surfeit Locus Protein 1 antibody
Anti-SURF1 antibody
Anti-SURF-1 antibody

Information sourced from Uniprot.org

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