• Western blot analysis of extracts of various cell lines, using Syntaxin 4 antibody (STJ25740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-STX4 antibody (1-275) (STJ25740)

SKU:
STJ25740

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STX4 (1-275) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX4
Gene ID: 6810
Uniprot ID: STX4_HUMAN
Immunogen Region: 1-275
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human Syntaxin 4 (NP_004595.2).
Immunogen Sequence: MRDRTHELRQGDDSSDEEDK ERVALVVHPGTARLGSPDEE FFHKVRTIRQTIVKLGNKVQ ELEKQQVTILATPLPEESMK QELQNLRDEIKQLGREIRLQ LKAIEPQKEEADENYNSVNT RMRKTQHGVLSQQFVELINK CNSMQSEYREKNVERIRRQL KITNAGMVSDEELEQMLDSG QSEVFVSNILKDTQVTRQAL NEISARHSEIQQLERSIREL HDIFTFLATEVEMQGEMIN
Tissue Specificity Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
Function Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.
Protein Name Syntaxin-4
Renal Carcinoma Antigen Ny-Ren-31
Database Links Reactome: R-HSA-114516
Reactome: R-HSA-1236974
Reactome: R-HSA-1445148
Reactome: R-HSA-199992
Reactome: R-HSA-449836
Cellular Localisation Cell Membrane
Single-Pass Type Iv Membrane Protein
Cell Projection
Neuron Projection
Localizes To Neurite Tips In Neuronal Cells
Alternative Antibody Names Anti-Syntaxin-4 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-31 antibody
Anti-STX4 antibody
Anti-STX4A antibody

Information sourced from Uniprot.org

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