• Western blot analysis of extracts of various cell lines, using StAR antibody (STJ25713) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g StAR antibody (STJ25713). Western blot was performed from the immunoprecipitate using StAR antibody (STJ25713) at a dilution of 1:1000.

Anti-STAR antibody (64-285) (STJ25713)

SKU:
STJ25713

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAR
Gene ID: 6770
Uniprot ID: STAR_HUMAN
Immunogen Region: 64-285
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 64-285 of human StAR (NP_000340.2).
Immunogen Sequence: EETLYSDQELAYLQQGEEAM QKALGILSNQEGWKKESQQD NGDKVMSKVVPDVGKVFRLE VVVDQPMERLYEELVERMEA MGEWNPNVKEIKVLQKIGKD TFITHELAAEAAGNLVGPRD FVSVRCAKRRGSTCVLAGMA TDFGNMPEQKGVIRAEHGPT CMVLHPLAGSPSKTKLTWLL SIDLKGWLPKSIINQVLSQT QVDFANHLRKRLESHPASEA RC
Tissue Specificity Expressed in gonads, adrenal cortex and kidney.
Function Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Protein Name Steroidogenic Acute Regulatory Protein - Mitochondrial
Star
Start Domain-Containing Protein 1
Stard1
Database Links Reactome: R-HSA-196108
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Steroidogenic Acute Regulatory Protein - Mitochondrial antibody
Anti-Star antibody
Anti-Start Domain-Containing Protein 1 antibody
Anti-Stard1 antibody
Anti-STAR antibody
Anti-STARD1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance