• Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (STJ110059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ST8SIA2 antibody (24-160) (STJ110059)

SKU:
STJ110059

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ST8SIA2 (24-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ST8SIA2
Gene ID: 8128
Uniprot ID: SIA8B_HUMAN
Immunogen Region: 24-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).
Immunogen Sequence: DISEIEEEIGNSGGRGTIRS AVNSLHSKSNRAEVVINGSS SPAVVDRSNESIKHNIQPAS SKWRHNQTLSLRIRKQILKF LDAEKDISVLKGTLKPGDII HYIFDRDSTMNVSQNLYELL PRTSPLKNKHFGTCAIV
Tissue Specificity Highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus.
Function May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.
Protein Name Alpha-2 -8-Sialyltransferase 8b
Sialyltransferase 8b
Siat8-B
Sialyltransferase St8sia Ii
St8siaii
Sialyltransferase X
Stx
Database Links Reactome: R-HSA-4085001
Reactome: R-HSA-419037
Reactome: R-HSA-975577
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Alpha-2 -8-Sialyltransferase 8b antibody
Anti-Sialyltransferase 8b antibody
Anti-Siat8-B antibody
Anti-Sialyltransferase St8sia Ii antibody
Anti-St8siaii antibody
Anti-Sialyltransferase X antibody
Anti-Stx antibody
Anti-ST8SIA2 antibody
Anti-SIAT8B antibody
Anti-STX antibody

Information sourced from Uniprot.org

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