• Western blot analysis of extracts of various cell lines, using SRPK1 antibody (STJ114384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using SRPK1 antibody (STJ114384). Blue: DAPI for nuclear staining.

Anti-SRPK1 antibody (290-480) (STJ114384)

SKU:
STJ114384

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRPK1 (290-480) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRPK1
Gene ID: 6732
Uniprot ID: SRPK1_HUMAN
Immunogen Region: 290-480
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-480 of human SRPK1 (NP_003128.3).
Immunogen Sequence: EEMEKESGPGQKRPNKQEES ESPVERPLKENPPNKMTQEK LEESSTIGQDQTLMERDTEG GAAEINCNGVIEVINYTQNS NNETLRHKEDLHNANDCDVQ NLNQESSFLSSQNGDSSTSQ ETDSCTPITSEVSDTMVCQS SSTVGQSFSEQHISQLQESI RAEIPCEDEQEQEHNGPLDN KGKSTAGNFLV
Tissue Specificity Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2. Highly expressed in different erythroid and lymphoid cell lines, with isoform 2 being far more abundant than isoform 1.
Function Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.
Protein Name Srsf Protein Kinase 1
Sfrs Protein Kinase 1
Serine/Arginine-Rich Protein-Specific Kinase 1
Sr-Protein-Specific Kinase 1
Database Links Reactome: R-HSA-9694631
Cellular Localisation Isoform 2: Cytoplasm
Nucleus
Nucleus Matrix
Microsome
Shuttles Between The Nucleus And The Cytoplasm
Inhibition Of The Hsp90 Atpase Activity
Osmotic Stress And Interaction With Hhv-1 Icp27 Protein Can Induce Its Translocation To The Nucleus
Kat5/Tip60 Inhibits Its Nuclear Translocation
Isoform 1: Cytoplasm
Mainly Localized In The Microsomal Fraction And The Cytoplasm
And To A Lesser Extent In The Nuclear Matrix
Cytoplasm
Nucleoplasm
Nucleus Speckle
Chromosome
Preferentially Localizes To The Promoter Of Gene Coding Regions
Alternative Antibody Names Anti-Srsf Protein Kinase 1 antibody
Anti-Sfrs Protein Kinase 1 antibody
Anti-Serine/Arginine-Rich Protein-Specific Kinase 1 antibody
Anti-Sr-Protein-Specific Kinase 1 antibody
Anti-SRPK1 antibody

Information sourced from Uniprot.org

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