• Western blot analysis of extracts of various cell lines, using SPTB antibody (STJ115015) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SPTB antibody (2080-2180) (STJ115015)

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STJ115015

€211.88 - €561.88
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPTB (2080-2180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPTB
Gene ID: 6710
Uniprot ID: SPTB1_HUMAN
Immunogen Region: 2080-2180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2080-2180 of human SPTB (NP_001020029.1).
Immunogen Sequence: QIAERPAEETGPQEEEGETA GEAPVSHHAATERTSPGEEE GTWPQNLQQPPPPGQHKDGQ KSTGDERPTTEPLFKVLDTP LSEGDEPATLPAPRDHGQSV Q
Post Translational Modifications The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110. (Microbial infection) Probably cleaved by P.falciparum SERA6.the cleavage results in SPTB solubilization causing the disruption of the actin cytoskeleton and the rupture of the erythrocyte cell membrane releasing the merozoites.
Function Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Protein Name Spectrin Beta Chain - Erythrocytic
Beta-I Spectrin
Database Links Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6807878
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Alternative Antibody Names Anti-Spectrin Beta Chain - Erythrocytic antibody
Anti-Beta-I Spectrin antibody
Anti-SPTB antibody
Anti-SPTB1 antibody

Information sourced from Uniprot.org

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