| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPRTN (1-240) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPRTN |
| Gene ID: |
83932 |
| Uniprot ID: |
SPRTN_HUMAN |
| Immunogen Region: |
1-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SPRTN (NP_001010984.1). |
| Immunogen Sequence: |
MDDDLMLALRLQEEWNLQEA ERDHAQESLSLVDASWELVD PTPDLQALFVQFNDQFFWGQ LEAVEVKWSVRMTLCAGICS YEGKGGMCSIRLSEPLLKLR PRKDLVETLLHEMIHAYLFV TNNDKDREGHGPEFCKHMHR INSLTGANITVYHTFHDEVD EYRRHWWRCNGPCQHRPPYY GYVKRATNREPSAHDYWWAE HQKTCGGTYIKIKEPENYSK KGKGKAKLGKEPVLAAENK |
| Post Translational Modifications | Autocatalytically cleaved in response to double-stranded DNA-binding: autocatalytic cleavage takes place in trans and leads to inactivation. Monoubiquitinated.monoubiquitination promotes exclusion from chromatin. Deubiquitinated by VCPIP1: deubiquitination is required for subsequent acetylation and recruitment to chromatin and DNA damage sites. Acetylated following deubiquitination by VCPIP1, leading to recruitment to chromatin and DNA damage sites. Phosphorylation by CHEK1 promotes recruitment to chromatin. |
| Function | DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4. Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. Involved in recruitment of VCP/p97 to sites of DNA damage. Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation. Does not activate CHEK1 in response to DNA damage. May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. |
| Protein Name | Dna-Dependent Metalloprotease SprtnDna Damage Protein Targeting VcpDvc1Protein With Sprt-Like Domain At The N TerminusSpartan |
| Database Links | Reactome: R-HSA-110320 |
| Cellular Localisation | NucleusChromosomeLocalizes To Sites Of Uv Damage Via The Pip-BoxRecruited To Stalled Replication Forks At Sites Of Replication Stress Following DeubiquitinationChek1 Stimulates Recruitment To Chromatin |
| Alternative Antibody Names | Anti-Dna-Dependent Metalloprotease Sprtn antibodyAnti-Dna Damage Protein Targeting Vcp antibodyAnti-Dvc1 antibodyAnti-Protein With Sprt-Like Domain At The N Terminus antibodyAnti-Spartan antibodyAnti-SPRTN antibodyAnti-C1orf124 antibodyAnti-DVC1 antibodyAnti-UNQ1880 antibodyAnti-PRO4323 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
