• Western blot analysis of lysates from U-87MG cells, using SPRTN Rabbit polyclonal antibody (STJ113225) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SPRTN antibody (1-240) (STJ113225)

SKU:
STJ113225

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPRTN (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPRTN
Gene ID: 83932
Uniprot ID: SPRTN_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SPRTN (NP_001010984.1).
Immunogen Sequence: MDDDLMLALRLQEEWNLQEA ERDHAQESLSLVDASWELVD PTPDLQALFVQFNDQFFWGQ LEAVEVKWSVRMTLCAGICS YEGKGGMCSIRLSEPLLKLR PRKDLVETLLHEMIHAYLFV TNNDKDREGHGPEFCKHMHR INSLTGANITVYHTFHDEVD EYRRHWWRCNGPCQHRPPYY GYVKRATNREPSAHDYWWAE HQKTCGGTYIKIKEPENYSK KGKGKAKLGKEPVLAAENK
Post Translational Modifications Autocatalytically cleaved in response to double-stranded DNA-binding: autocatalytic cleavage takes place in trans and leads to inactivation. Monoubiquitinated.monoubiquitination promotes exclusion from chromatin. Deubiquitinated by VCPIP1: deubiquitination is required for subsequent acetylation and recruitment to chromatin and DNA damage sites. Acetylated following deubiquitination by VCPIP1, leading to recruitment to chromatin and DNA damage sites. Phosphorylation by CHEK1 promotes recruitment to chromatin.
Function DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity. DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde. Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis. Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4. Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs. SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs. Involved in recruitment of VCP/p97 to sites of DNA damage. Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation. Does not activate CHEK1 in response to DNA damage. May also act as a 'reader' of ubiquitinated PCNA: recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis.
Protein Name Dna-Dependent Metalloprotease Sprtn
Dna Damage Protein Targeting Vcp
Dvc1
Protein With Sprt-Like Domain At The N Terminus
Spartan
Database Links Reactome: R-HSA-110320
Cellular Localisation Nucleus
Chromosome
Localizes To Sites Of Uv Damage Via The Pip-Box
Recruited To Stalled Replication Forks At Sites Of Replication Stress Following Deubiquitination
Chek1 Stimulates Recruitment To Chromatin
Alternative Antibody Names Anti-Dna-Dependent Metalloprotease Sprtn antibody
Anti-Dna Damage Protein Targeting Vcp antibody
Anti-Dvc1 antibody
Anti-Protein With Sprt-Like Domain At The N Terminus antibody
Anti-Spartan antibody
Anti-SPRTN antibody
Anti-C1orf124 antibody
Anti-DVC1 antibody
Anti-UNQ1880 antibody
Anti-PRO4323 antibody

Information sourced from Uniprot.org

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