| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPOP (1-270) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Spop |
| Gene ID: |
20747 |
| Uniprot ID: |
SPOP_MOUSE |
| Immunogen Region: |
1-270 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of mouse SPOP (NP_079563.2). |
| Immunogen Sequence: |
MSRVPSPPPPAEMSSGPVAE SWCYTQIKVVKFSYMWTINN FSFCREEMGEVIKSSTFSSG ANDKLKWCLRVNPKGLDEES KDYLSLYLLLVSCPKSEVRA KFKFSILNAKGEETKAMESQ RAYRFVQGKDWGFKKFIRRD FLLDEANGLLPDDKLTLFCE VSVVQDSVNISGQNTMNMVK VPECRLADELGGLWENSRFT DCCLCVAGQEFQAHKAILAA RSPVFSAMFEHEMEESKKN |
| Tissue Specificity | Widely expressed, mainly in pancreas and in particular in adult pancreatic insulin-producing beta cells and in a subset of exocrine acinar and duct cells. |
| Function | Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1.this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability. |
| Protein Name | Speckle-Type Poz ProteinHib Homolog 1Pdx-1 C-Terminal-Interacting Factor 1 |
| Database Links | Reactome: R-MMU-5632684 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-Speckle-Type Poz Protein antibodyAnti-Hib Homolog 1 antibodyAnti-Pdx-1 C-Terminal-Interacting Factor 1 antibodyAnti-Spop antibodyAnti-Pcif1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
