• Western blot analysis of various lysates using SPOP Rabbit polyclonal antibody (STJ114000) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-SPOP antibody (220-374) (STJ114000)

SKU:
STJ114000

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPOP (220-374) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPOP
Gene ID: 8405
Uniprot ID: SPOP_HUMAN
Immunogen Region: 220-374
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-374 of human SPOP (NP_001007227.1).
Immunogen Sequence: ARSPVFSAMFEHEMEESKKN RVEINDVEPEVFKEMMCFIY TGKAPNLDKMADDLLAAADK YALERLKVMCEDALCSNLSV ENAAEILILADLHSADQLKT QAVDFINYHASDVLETSGWK SMVVSHPHLVAEAYRSLASA QCPFLGPPRKRLKQS
Tissue Specificity Widely expressed.
Function Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1.this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. Involved in the regulation of bromodomain and extra-terminal motif (BET) proteins BRD2, BRD3, BRD4 stability.
Protein Name Speckle-Type Poz Protein
Hib Homolog 1
Roadkill Homolog 1
Database Links Reactome: R-HSA-5632684
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Speckle-Type Poz Protein antibody
Anti-Hib Homolog 1 antibody
Anti-Roadkill Homolog 1 antibody
Anti-SPOP antibody

Information sourced from Uniprot.org

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