• Western blot analysis of various lysates using SPG20 Rabbit polyclonal antibody (STJ119706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SPG20 antibody (1-350) (STJ119706)

SKU:
STJ119706

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPG20 (1-350) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPART
Gene ID: 23111
Uniprot ID: SPART_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human SPG20 (NP_055902.1).
Immunogen Sequence: MEQEPQNGEPAEIKIIREAY KKAFLFVNKGLNTDELGQKE EAKNYYKQGIGHLLRGISIS SKESEHTGPGWESARQMQQK MKETLQNVRTRLEILEKGLA TSLQNDLQEVPKLYPEFPPK DMCEKLPEPQSFSSAPQHAE VNGNTSTPSAGAVAAPASLS LPSQSCPAEAPPAYTPQAAE GHYTVSYGTDSGEFSSVGEE FYRNHSQPPPLETLGLDADE LILIPNGVQIFFVNPAGEV
Tissue Specificity Ubiquitously expressed, with highest levels of expression detected in adipose tissue.
Post Translational Modifications Ubiquitinated.ubiquitination does not require ITCH and WWP1.
Function May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.
Protein Name Spartin
Spastic Paraplegia 20 Protein
Trans-Activated By Hepatitis C Virus Core Protein 1
Cellular Localisation Cytoplasm
Midbody
Transiently Associated With Endosomes
Colocalized With Ist1 To The Ends Of Flemming Bodies During Cytokinesis
Alternative Antibody Names Anti-Spartin antibody
Anti-Spastic Paraplegia 20 Protein antibody
Anti-Trans-Activated By Hepatitis C Virus Core Protein 1 antibody
Anti-SPART antibody
Anti-KIAA0610 antibody
Anti-SPG20 antibody
Anti-TAHCCP1 antibody

Information sourced from Uniprot.org

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