• Western blot analysis of extracts of A549 cells, using SPDYA antibody (STJ115567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-SPDYA antibody (1-313) (STJ115567)

SKU:
STJ115567

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPDYA (1-313) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPDYA
Gene ID: 245711
Uniprot ID: SPDYA_HUMAN
Immunogen Region: 1-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_877433.2).
Immunogen Sequence: MRHNQMCCETPPTVTVYVKS GSNRSHQPKKPITLKRPICK DNWQAFEKNTHNNNKSKRPK GPCLVIQRQDMTAFFKLFDD DLIQDFLWMDCCCKIADKYL LAMTFVYFKRAKFTISEHTR INFFIALYLANTVEEDEEET KYEIFPWALGKNWRKLFPNF LKLRDQLWDRIDYRAIVSRR CCEEVMAIAPTHYIWQRERS VHHSGAVRNYNRDEVQLPRG PSATPVDCSLCGKKRRYVR
Tissue Specificity Highly expressed in testis. Expressed at a low level in wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, salivary gland, thyroid gland, thymus, trachea and uterus. Expressed at a slightly higher level in adrenal gland, cerebellum, small intestine, lung, prostate and trachea. Expression is cell cycle-dependent, being restricted to cells in G1/S phase.
Function Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Mediates cell survival during the DNA damage process through activation of CDK2.
Protein Name Speedy Protein A
Rapid Inducer Of G2/M Progression In Oocytes A
Ringo A
Hspy/Ringo A
Speedy-1
Spy1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Speedy Protein A antibody
Anti-Rapid Inducer Of G2/M Progression In Oocytes A antibody
Anti-Ringo A antibody
Anti-Hspy/Ringo A antibody
Anti-Speedy-1 antibody
Anti-Spy1 antibody
Anti-SPDYA antibody
Anti-SPDY1 antibody
Anti-SPY1 antibody

Information sourced from Uniprot.org

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