| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPAST (120-325) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPAST |
| Gene ID: |
6683 |
| Uniprot ID: |
SPAST_HUMAN |
| Immunogen Region: |
120-325 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-325 of human SPAST (NP_055761.2). |
| Immunogen Sequence: |
HKQAFEYISIALRIDEDEKA GQKEQAVEWYKKGIEELEKG IAVIVTGQGEQCERARRLQA KMMTNLVMAKDRLQLLEKMQ PVLPFSKSQTDVYNDSTNLA CRNGHLQSESGAVPKRKDPL THTSNSLPRSKTVMKTGSAG LSGHHRAPSYSGLSMVSGVK QGSGPAPTTHKGTPKTNRTN KPSTPTTATRKKKDLKNFRN VDSNLA |
| Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The short isoforms may predominate in brain and spinal cord. |
| Function | ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex. Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex. Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling. Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing. Probably plays a role in axon growth and the formation of axonal branches. Isoform 1: Involved in lipid metabolism by regulating the size and distribution of lipid droplets. |
| Protein Name | SpastinSpastic Paraplegia 4 Protein |
| Database Links | Reactome: R-HSA-9668328 |
| Cellular Localisation | MembranePeripheral Membrane ProteinEndoplasmic ReticulumMidbodyCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomePerinuclear RegionNucleusSpindleCell ProjectionAxonForms An Intramembrane Hairpin-Like Structure In The MembraneLocalization To The Centrosome Is Independent Of MicrotubulesLocalizes To The Midbody Of Dividing CellsAnd This Requires Chmp1bEnriched In The Distal Axons And Branches Of Postmitotic NeuronsMainly Nuclear In Interphase Cells And Becomes Associated With The CentrosomesSpindle MicrotubulesMidzone And Finally The Midbody During Cell DivisionIsoform 1: Endoplasmic Reticulum MembraneNucleus MembraneLipid DropletEndosomeRecruited To Nuclear Membrane By Ist1 During Late AnaphaseLocalizes To Endoplasmic Reticulum Tubular NetworkIsoform 3: CytoplasmConstitutes The Main Endosomal Form |
| Alternative Antibody Names | Anti-Spastin antibodyAnti-Spastic Paraplegia 4 Protein antibodyAnti-SPAST antibodyAnti-ADPSP antibodyAnti-FSP2 antibodyAnti-KIAA1083 antibodyAnti-SPG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
