• Immunoprecipitation of SOD1 in 300 µg extracts from MCF7 cells using 3 µg SOD1 Rabbit polyclonal antibody (STJ25653). Western blot analysis was performed using SOD1 Rabbit polyclonal antibody (STJ25653) at 1:1000 dilution.
  • Immunofluorescence analysis of NIH/3T3 cells using SOD1 Rabbit polyclonal antibody (STJ25653) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SOD1 in paraffin-embedded rat kidney using SOD1 Rabbit polyclonal antibody (STJ25653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SOD1 in paraffin-embedded human colon carcinoma using SOD1 Rabbit polyclonal antibody (STJ25653) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SOD1 Rabbit polyclonal antibody (STJ25653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SOD1 antibody (2-154) (STJ25653)

SKU:
STJ25653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SOD1 (2-154) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOD1
Gene ID: 6647
Uniprot ID: SODC_HUMAN
Immunogen Region: 2-154
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-154 of human SOD1 (NP_000445.1).
Immunogen Sequence: ATKAVCVLKGDGPVQGIINF EQKESNGPVKVWGSIKGLTE GLHGFHVHEFGDNTAGCTSA GPHFNPLSRKHGGPKDEERH VGDLGNVTADKDGVADVSIE DSVISLSGDHCIIGRTLVVH EKADDLGKGGNEESTKTGNA GSRLACGVIGIAQ
Post Translational Modifications Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.
Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name Superoxide Dismutase Cu-Zn
Superoxide Dismutase 1
Hsod1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3299685
Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Predominantly Cytoplasmic
The Pathogenic Variants Als1 Arg-86 And Ala-94 Gradually Aggregates And Accumulates In Mitochondria
Alternative Antibody Names Anti-Superoxide Dismutase Cu-Zn antibody
Anti-Superoxide Dismutase 1 antibody
Anti-Hsod1 antibody
Anti-SOD1 antibody

Information sourced from Uniprot.org

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