Western blot analysis of Rat brain, using SNAP29 antibody (STJ25638) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SNAP29 antibody (1-258) (STJ25638)

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STJ25638
$151.88 - $708.08
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNAP29
Gene ID: 9342
Uniprot ID: SNP29_HUMAN
Immunogen Region: 1-258
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human SNAP29 (NP_004773.1).
Immunogen Sequence: MSAYPKSYNPFDDDGEDEGA RPAPWRDARDLPDGPDAPAD RQQYLRQEVLRRAEATAAST SRSLALMYESEKVGVASSEE LARQRGVLERTEKMVDKMDQ DLKISQKHINSIKSVFGGLV NYFKSKPVETPPEQNGTLTS QPNNRLKEAISTSKEQEAKY QASHPNLRKLDDTDPVPRGA GSAMSTDAYPKNPHLRAYHQ KIDSNLDELSMGLGRLKDIA LGMQTEIEEQDDILDRLTT
Tissue Specificity Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.
Protein Name Synaptosomal-Associated Protein 29
Snap-29
Soluble 29 Kda Nsf Attachment Protein
Vesicle-Membrane Fusion Protein Snap-29
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6811438
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Vesicle
Autophagosome Membrane
Cell Projection
Cilium Membrane
Appears To Be Mostly Membrane-Bound
Probably Via Interaction With Syntaxins
But A Significant Portion Is Cytoplasmic
Localizes To The Ciliary Pocket From Where The Cilium Protrudes
Alternative Antibody Names Anti-Synaptosomal-Associated Protein 29 antibody
Anti-Snap-29 antibody
Anti-Soluble 29 Kda Nsf Attachment Protein antibody
Anti-Vesicle-Membrane Fusion Protein Snap-29 antibody
Anti-SNAP29 antibody

Information sourced from Uniprot.org

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