• Immunohistochemistry analysis of paraffin-embedded human colon using Snail Rabbit polyclonal antibody (STJ114189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Snail antibody (STJ114189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SNAI1 antibody (1-264) (STJ114189)

SKU:
STJ114189

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Snail (1-264) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNAI1
Gene ID: 6615
Uniprot ID: SNAI1_HUMAN
Immunogen Region: 1-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human Snail (O95863).
Immunogen Sequence: MPRSFLVRKPSDPNRKPNYS ELQDSNPEFTFQQPYDQAHL LAAIPPPEILNPTASLPMLI WDSVLAPQAQPIAWASLRLQ ESPRVAELTSLSDEDSGKGS QPPSPPSPAPSSFSSTSVSS LEAEAYAAFPGLGQVPKQLA QLSEAKDLQARKAFNCKYCN KEYLSLGALKMHIRSHTLPC VCGTCGKAFSRPWLLQGHVR THTGEKPFSCPHCSRAFADR SNLRAHLQTHSDVKKYQCQ
Tissue Specificity Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Post Translational Modifications Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2. Ubiquitinated in a FBXL5-dependent manner.preventing interaction with DNA and promoting its degradation. Deubiquitinated by USP37.leading to stabilization. O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein. ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
Function Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Protein Name Zinc Finger Protein Snai1
Protein Snail Homolog 1
Protein Sna
Database Links Reactome: R-HSA-8943724
Reactome: R-HSA-9758919
Reactome: R-HSA-9762293
Cellular Localisation Nucleus
Cytoplasm
Once Phosphorylated (Probably On Ser-107
Ser-111
Ser-115 And Ser-119) It Is Exported From The Nucleus To The Cytoplasm Where Subsequent Phosphorylation Of The Destruction Motif And Ubiquitination Involving Btrc Occurs
Alternative Antibody Names Anti-Zinc Finger Protein Snai1 antibody
Anti-Protein Snail Homolog 1 antibody
Anti-Protein Sna antibody
Anti-SNAI1 antibody
Anti-SNAH antibody

Information sourced from Uniprot.org

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