| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SMARCAD1 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMARCAD1 |
| Gene ID: |
56916 |
| Uniprot ID: |
SMRCD_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMARCAD1 (NP_001121901.1). |
| Immunogen Sequence: |
MNLFNLDRFRFEKRNKIEEA PEATPQPSQPGPSSPISLSA EEENAEGEVSRANTPDSDIT EKTEDSSVPETPDNERKASI SYFKNQRGIQYIDLSSDSED VVSPNCSNTVQEKTFNKDTV IIVSEPSEDEESQGLPTMAR RNDDISELEDLSELEDLKDA KLQTLKELFPQRSDNDLLKL IESTSTMDGAIAAALLMFGD AGGGPRKRKLSSSSEPYEED EFNDDQSIKKTRLDHGEES |
| Tissue Specificity | Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level). |
| Function | DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing. |
| Protein Name | Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1Atp-Dependent Helicase 1Hhel1 |
| Cellular Localisation | NucleusChromosomeColocalizes With Pcna At Replication Forks During S PhaseRecruited To Double-Strand Breaks (Dsbs) Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1 antibodyAnti-Atp-Dependent Helicase 1 antibodyAnti-Hhel1 antibodyAnti-SMARCAD1 antibodyAnti-KIAA1122 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")