• Western blot analysis of extracts of various cell lines, using SLC7A2 antibody (STJ116784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human mammary cancer using SLC7A2 antibody (STJ116784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using SLC7A2 antibody (STJ116784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using SLC7A2 antibody (STJ116784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using SLC7A2 antibody (STJ116784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SLC7A2 antibody (642-658) (STJ116784)

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STJ116784

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC7A2 (642-658) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC7A2
Gene ID: 6542
Uniprot ID: CTR2_HUMAN
Immunogen Region: 642-658
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 642-658 of human SLC7A2 (NP_001008539.3).
Immunogen Sequence: PRNLSSPFIFHEKTSEF
Tissue Specificity Expressed at high levels in the skeletal muscle, placenta and ovary. Expressed at intermediate levels in the liver and pancreas and at low levels in the kidney and heart.
Function Functions as permease involved in the transport of the cationic amino acids (L-arginine, L-lysine, L-ornithine and L-homoarginine).the affinity for its substrates differs between isoforms created by alternative splicing. May play a role in classical or alternative activation of macrophages via its role in arginine transport. Isoform 1: Functions as permease that mediates the transport of the cationic amino acids (L-arginine, L-lysine, L-ornithine and L-homoarginine). Shows a much higher affinity for L-arginine and L-homoarginine than isoform 2. Isoform 2: Functions as low-affinity, high capacity permease involved in the transport of the cationic amino acids (L-arginine, L-lysine, L-ornithine and L-homoarginine).
Protein Name Cationic Amino Acid Transporter 2
Cat-2
Cat2
Low Affinity Cationic Amino Acid Transporter 2
Solute Carrier Family 7 Member 2
Database Links Reactome: R-HSA-352230
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cationic Amino Acid Transporter 2 antibody
Anti-Cat-2 antibody
Anti-Cat2 antibody
Anti-Low Affinity Cationic Amino Acid Transporter 2 antibody
Anti-Solute Carrier Family 7 Member 2 antibody
Anti-SLC7A2 antibody
Anti-ATRC2 antibody
Anti-CAT2 antibody

Information sourced from Uniprot.org

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