• Immunohistochemistry analysis of paraffin-embedded human stomach using SLC30A1 antibody (STJ114406) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using SLC30A1 antibody (STJ114406) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SLC30A1 antibody (STJ114406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC30A1 antibody (251-350) (STJ114406)

SKU:
STJ114406

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC30A1 (251-350) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC30A1
Gene ID: 7779
Uniprot ID: ZNT1_HUMAN
Immunogen Region: 251-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 251-350 of human SLC30A1 (NP_067017.2).
Immunogen Sequence: HVLGDALGSVIVVVNALVFY FSWKGCSEGDFCVNPCFPDP CKAFVEIINSTHASVYEAGP CWVLYLDPTLCVVMVCILLY TTYPLLKESALILLQTVPKQ
Post Translational Modifications N-glycosylated at Asn-299. N-glycosylation promotes endocytosis and degradation through the proteasomal or lysosomal pathways.
Function Zinc ion:proton antiporter that could function at the plasma membrane mediating zinc efflux from cells against its electrochemical gradient protecting them from intracellular zinc accumulation and toxicity. Alternatively, could prevent the transport to the plasma membrane of CACNB2, the L-type calcium channels regulatory subunit, through a yet to be defined mechanism. By modulating the expression of these channels at the plasma membrane, could prevent calcium and zinc influx into cells. By the same mechanism, could also prevent L-type calcium channels-mediated heavy metal influx into cells. In some cells, could also function as a zinc ion:proton antiporter mediating zinc entry into the lumen of cytoplasmic vesicles. In macrophages, can increase zinc ions concentration into the lumen of cytoplasmic vesicles containing engulfed bacteria and could help inactivate them.
Protein Name Proton-Coupled Zinc Antiporter Slc30a1
Solute Carrier Family 30 Member 1
Zinc Transporter 1
Database Links Reactome: R-HSA-435368
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cytoplasmic Vesicle Membrane
Localization To The Plasma Membrane Is Regulated By Cellular Zinc Status
Recruitment To The Plasma Membrane From An Internal Pool Is Stimulated By Zinc While In Absence Of Zinc The Plasma Membrane Pool Is Endocytosed And Degraded
Localizes To The Basolateral Surface Of Enterocytes
Localizes To Zinc-Containing Intracellular Vesicles In Macrophages
Alternative Antibody Names Anti-Proton-Coupled Zinc Antiporter Slc30a1 antibody
Anti-Solute Carrier Family 30 Member 1 antibody
Anti-Zinc Transporter 1 antibody
Anti-SLC30A1 antibody
Anti-ZNT1 antibody

Information sourced from Uniprot.org

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