• Western blot analysis of extracts of various cell lines, using GLUT4 antibody (STJ29952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using GLUT4 antibody (STJ29952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using GLUT4 rabbit polyclonal antibody (STJ29952) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse skeletal muscle using GLUT4 antibody (STJ29952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of RD cells using GLUT4 antibody (STJ29952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C2C12 cells using GLUT4 antibody (STJ29952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Anti-SLC2A4 antibody (450-509) (STJ29952)

Anti-SLC2A4 antibody (450-509) (STJ29952)

SKU:
STJ29952

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLUT4 (450-509) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A4
Gene ID: 6517
Uniprot ID: GLUT4_HUMAN
Immunogen Region: 450-509
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-509 of human GLUT4 (NP_001033.1).
Immunogen Sequence: FLLFAVLLLGFFIFTFLRVP ETRGRTFDQISAAFHRTPSL LEQEVKPSTELEYLGPDEND
Tissue Specificity Skeletal and cardiac muscles.brown and white fat.
Post Translational Modifications Sumoylated. Palmitoylated. Palmitoylation by ZDHHC7 controls the insulin-dependent translocation of GLUT4 to the plasma membrane.
Function Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4
Glucose Transporter Type 4 - Insulin-Responsive
Glut-4
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-189200
Reactome: R-HSA-381340
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endomembrane System
Cytoplasm
Perinuclear Region
Localizes Primarily To The Perinuclear Region
Undergoing Continued Recycling To The Plasma Membrane Where It Is Rapidly Reinternalized
The Dileucine Internalization Motif Is Critical For Intracellular Sequestration
Insulin Stimulation Induces Translocation To The Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 4 antibody
Anti-Glucose Transporter Type 4 - Insulin-Responsive antibody
Anti-Glut-4 antibody
Anti-SLC2A4 antibody
Anti-GLUT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance