• Western blot analysis of extracts of various cell lines, using SLC17A6 antibody (STJ116420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SLC17A6 antibody (483-582) (STJ116420)

SKU:
STJ116420

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC17A6 (483-582) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC17A6
Gene ID: 57084
Uniprot ID: VGLU2_HUMAN
Immunogen Region: 483-582
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 483-582 of human SLC17A6 (NP_065079.1).
Immunogen Sequence: AALVHYGGVIFYAIFASGEK QPWADPEETSEEKCGFIHED ELDEETGDITQNYINYGTTK SYGATTQANGGWPSGWEKKE EFVQGEVQDSHSYKDRVDYS
Tissue Specificity Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
Function Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as a uniporter which transports preferentially L-glutamate but also, phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows the chloride permeation through the synaptic vesicle membrane therefore affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, functions as a vesicular K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular H(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (Probable). The symporter activity is driven by an inside negative membrane potential and is electrogenic (Probable). Also involved in the regulation of retinal hyaloid vessel regression during postnatal development. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas.
Protein Name Vesicular Glutamate Transporter 2
Vglut2
Differentiation-Associated Bnpi
Differentiation-Associated Na(+-Dependent Inorganic Phosphate Cotransporter
Solute Carrier Family 17 Member 6
Database Links Reactome: R-HSA-428643
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Cell Membrane
Alternative Antibody Names Anti-Vesicular Glutamate Transporter 2 antibody
Anti-Vglut2 antibody
Anti-Differentiation-Associated Bnpi antibody
Anti-Differentiation-Associated Na(+-Dependent Inorganic Phosphate Cotransporter antibody
Anti-Solute Carrier Family 17 Member 6 antibody
Anti-SLC17A6 antibody
Anti-DNPI antibody
Anti-VGLUT2 antibody

Information sourced from Uniprot.org

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