• Western blot analysis of various lysates using SLC12A3 Rabbit polyclonal antibody (STJ11100335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC12A3 antibody (1-140) (STJ11100335)

SKU:
STJ11100335

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC12A3 (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC12A3
Gene ID: 6559
Uniprot ID: S12A3_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SLC12A3 (NP_000330.2).
Immunogen Sequence: MAELPTTETPGDATLCSGRF TISTLLSSDEPSPPAAYDSS HPSHLTHSSTFCMRTFGYNT IDVVPTYEHYANSTQPGEPR KVRPTLADLHSFLKQEGRHL HALAFDSRPSHEMTDGLVEG EAGTSSEKNPEEPVRFGWVK
Tissue Specificity Predominantly expressed in the kidney (at protein level). Localizes to the distal convoluted tubules (at protein level). Not detected in normal aorta, but abundantly expressed in fatty streaks and advanced atherosclerotic lesions (at protein level).
Post Translational Modifications Ubiquitinated.ubiquitination is essential for regulation of endocytosis. The BCR(KLHL3) complex was initially identified as a candidate ubiquitin ligase for SLC12A3. However, it was later shown that it is not the case. Phosphorylated at Thr-46, Thr-55, Thr-60 and Ser-91 by OXSR1/OSR1 and STK39/SPAK downstream of WNK4, promoting its activity. Phosphorylated in response to IL18.
Function Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.
Protein Name Solute Carrier Family 12 Member 3
Na-Cl Cotransporter
Ncc
Na-Cl Symporter
Thiazide-Sensitive Sodium-Chloride Cotransporter
Database Links Reactome: R-HSA-426117
Reactome: R-HSA-5619087
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 3 antibody
Anti-Na-Cl Cotransporter antibody
Anti-Ncc antibody
Anti-Na-Cl Symporter antibody
Anti-Thiazide-Sensitive Sodium-Chloride Cotransporter antibody
Anti-SLC12A3 antibody
Anti-NCC antibody
Anti-TSC antibody

Information sourced from Uniprot.org

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