• Western blot analysis of extracts from normal (control) and SIPA1 knockout (KO) HeLa cells, using SIPA1 antibody (STJ11100743) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SIPA1 antibody (763-1042) (STJ11100743)

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STJ11100743

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIPA1 (763-1042) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIPA1
Gene ID: 6494
Uniprot ID: SIPA1_HUMAN
Immunogen Region: 763-1042
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 763-1042 of human SIPA1 (NP_694985.29).
Immunogen Sequence: ESGRPRRSFSELYTLSLQEP SRRGAPDPVQDEVQGVTLLP TTKQLLHLCLQDGGSPPGPG DLAEERTEFLHSQNSLSPRS SLSDEAPVLPNTTPDLLLAT TAKPSVPSADSETPLTQDRP GSPSGSEDKGNPAPELRASF LPRTLSLRNSISRIMSEAGS GTLEDEWQAISEIASTCNTI LESLSREGQPIPESGDPKGT PKSDAEPEPGNLSEKVSHLE SMLRKLQEDLQKEKADRAA
Tissue Specificity Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
Function GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state. Affects cell cycle progression.
Protein Name Signal-Induced Proliferation-Associated Protein 1
Sipa-1
Gtpase-Activating Protein Spa-1
P130 Spa-1
Database Links Reactome: R-HSA-392517
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Endomembrane System
Peripheral Membrane Protein
Mostly Localized In The Perinuclear Membraneous Region
Alternative Antibody Names Anti-Signal-Induced Proliferation-Associated Protein 1 antibody
Anti-Sipa-1 antibody
Anti-Gtpase-Activating Protein Spa-1 antibody
Anti-P130 Spa-1 antibody
Anti-SIPA1 antibody
Anti-SPA1 antibody

Information sourced from Uniprot.org

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