• Western blot analysis of extracts of K-562 cells, using Siglec-15 antibody (STJ11103445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human tonsil using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using Siglec-15 rabbit monoclonal antibody (STJ11103445) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Siglec-15 antibody (20-263) [S5MR] (STJ11103445)

SKU:
STJ11103445

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Siglec-15 (20-263) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIGLEC15
Gene ID: 284266
Uniprot ID: SIG15_HUMAN
Immunogen Region: 20-263
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-263 of human Siglec-15 (NP_998767.1).
Immunogen Sequence: FVRTKIDTTENLLNTEVHSS PAQRWSMQVPPEVSAEAGDA AVLPCTFTHPHRHYDGPLTA IWRAGEPYAGPQVFRCAAAR GSELCQTALSLHGRFRLLGN PRRNDLSLRVERLALADDRR YFCRVEFAGDVHDRYESRHG VRLHVTAAPRIVNISVLPSP AHAFRALCTAEGEPPPALAW SGPALGNSLAAVRSPREGHG HLVTAELPALTHDGRYTCTA ANSLGRSEASVYLFRFHGA
Tissue Specificity Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.
Function Binds sialylated glycoproteins.
Protein Name Sialic Acid-Binding Ig-Like Lectin 15
Siglec-15
Cd33 Antigen-Like 3
Database Links Reactome: R-HSA-2172127
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Sialic Acid-Binding Ig-Like Lectin 15 antibody
Anti-Siglec-15 antibody
Anti-Cd33 Antigen-Like 3 antibody
Anti-SIGLEC15 antibody
Anti-CD33L3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance