• Western blot analysis of extracts of various cell lines, using SERTAD1 antibody (STJ116321) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-SERTAD1 antibody (1-236) (STJ116321)

SKU:
STJ116321

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERTAD1 (1-236) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERTAD1
Gene ID: 29950
Uniprot ID: SRTD1_HUMAN
Immunogen Region: 1-236
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of human SERTAD1 (NP_037508.2).
Immunogen Sequence: MLSKGLKRKREEEEEKEPLA VDSWWLDPGHTAVAQAPPAV ASSSLFDLSVLKLHHSLQQS EPDLRHLVLVVNTLRRIQAS MAPAAALPPVPSPPAAPSVA DNLLASSDAALSASMASLLE DLSHIEGLSQAPQPLADEGP PGRSIGGAAPSLGALDLLGP ATGCLLDDGLEGLFEDIDTS MYDNELWAPASEGLKPGPED GPGKEEAPELDEAELDYLMD VLVGTQALERPPGPGR
Post Translational Modifications Polyubiquitinated, which promotes proteasomal degradation.
Function Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. Stimulates E2F1/TFDP1 transcriptional activity. Renders the activity of cyclin D1/CDK4 resistant to the inhibitory effects of CDKN2A/p16INK4A.
Protein Name Serta Domain-Containing Protein 1
Cdk4-Binding Protein P34sei1
Sei-1
P34(Sei-1
Transcriptional Regulator Interacting With The Phd-Bromodomain 1
Trip-Br1
Alternative Antibody Names Anti-Serta Domain-Containing Protein 1 antibody
Anti-Cdk4-Binding Protein P34sei1 antibody
Anti-Sei-1 antibody
Anti-P34(Sei-1 antibody
Anti-Transcriptional Regulator Interacting With The Phd-Bromodomain 1 antibody
Anti-Trip-Br1 antibody
Anti-SERTAD1 antibody
Anti-SEI1 antibody
Anti-TRIPBR1 antibody

Information sourced from Uniprot.org

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