| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SERPINB5 (21-189) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SERPINB5 |
| Gene ID: |
5268 |
| Uniprot ID: |
SPB5_HUMAN |
| Immunogen Region: |
21-189 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 21-189 of human SERPINB5 (NP_002630.2). |
| Immunogen Sequence: |
EKEPLGNVLFSPICLSTSLS LAQVGAKGDTANEIGQVLHF ENVKDVPFGFQTVTSDVNKL SSFYSLKLIKRLYVDKSLNL STEFISSTKRPYAKELETVD FKDKLEETKGQINNSIKDLT DGHFENILADNSVNDQTKIL VVNAAYFVGKWMKKFSESET KECPFRVNK |
| Tissue Specificity | Normal mammary epithelial cells. |
| Function | Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Protein Name | Serpin B5MaspinPeptidase Inhibitor 5Pi-5 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Serpin B5 antibodyAnti-Maspin antibodyAnti-Peptidase Inhibitor 5 antibodyAnti-Pi-5 antibodyAnti-SERPINB5 antibodyAnti-PI5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/100497/387047/STJ25485_1__70610.1713156238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ11100724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89662/358159/STJ11100724_1__70225.1713122460.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ11100724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
