• Western blot analysis of extracts of various cell lines, using Septin 7 antibody (STJ28963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human lung cancer using Septin 7 antibody (STJ28963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse Intestine using Septin 7 antibody (STJ28963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Septin 7 antibody (STJ28963) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SEPT7 antibody (307-436) (STJ28963)

SKU:
STJ28963

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEPT7 (307-436) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN7
Gene ID: 989
Uniprot ID: SEPT7_HUMAN
Immunogen Region: 307-436
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-436 of human Septin 7 (NP_001011553.2).
Immunogen Sequence: NYRSRKLAAVTYNGVDNNKN KGQLTKSPLAQMEEERREHV AKMKKMEMEMEQVFEMKVKE KVQKLKDSEAELQRRHEQMK KNLEAQHKELEEKRRQFEDE KANWEAQQRILEQQNSSRTL EKNKKKGKIF
Tissue Specificity Widely expressed.
Function Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.
Protein Name Septin-7
Cdc10 Protein Homolog
Database Links Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Chromosome
Centromere
Kinetochore
Cytoskeleton
Spindle
Cleavage Furrow
Midbody
Cilium Axoneme
Cell Projection
Cilium
Flagellum
Distributed Throughout The Cytoplasm In Prometaphase Cells
Associated With The Spindle During Metaphase
Associated With The Central Spindle And At The Cleavage Furrow In Anaphase Cells
Detected At The Midbody In Telophase
Associated With Actin Stress Fibers
Found In The Sperm Annulus
Alternative Antibody Names Anti-Septin-7 antibody
Anti-Cdc10 Protein Homolog antibody
Anti-SEPTIN7 antibody
Anti-CDC10 antibody
Anti-SEPT7 antibody

Information sourced from Uniprot.org

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