| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SEPHS1 (1-392) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:10-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SEPHS1 |
| Gene ID: |
22929 |
| Uniprot ID: |
SPS1_HUMAN |
| Immunogen Region: |
1-392 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2). |
| Immunogen Sequence: |
MSTRESFNPESYELDKSFRL TRFTELKGTGCKVPQDVLQK LLESLQENHFQEDEQFLGAV MPRLGIGMDTCVIPLRHGGL SLVQTTDYIYPIVDDPYMMG RIACANVLSDLYAMGVTECD NMLMLLGVSNKMTDRERDKV MPLIIQGFKDAAEEAGTSVT GGQTVLNPWIVLGGVATTVC QPNEFIMPDNAVPGDVLVLT KPLGTQVAVAVHQWLDIPEK WNKIKLVVTQEDVELAYQE |
| Tissue Specificity | Isoform 1: Gradually expressed during the cell cycle until G2/M phase and then decreases. Isoform 2: Gradually expressed during the cell cycle until G2/M phase and then decreases. Isoform 3: Gradually expressed during the cell cycle until S phase and then decreases. |
| Function | Synthesizes selenophosphate from selenide and ATP. |
| Protein Name | Selenide - Water Dikinase 1Selenium Donor Protein 1Selenophosphate Synthase 1 |
| Cellular Localisation | Isoform 1: Cell MembranePeripheral Membrane ProteinNucleus MembraneIsoform 2: CytoplasmIsoform 3: CytoplasmIsoform 4: Cytoplasm |
| Alternative Antibody Names | Anti-Selenide - Water Dikinase 1 antibodyAnti-Selenium Donor Protein 1 antibodyAnti-Selenophosphate Synthase 1 antibodyAnti-SEPHS1 antibodyAnti-SELD antibodyAnti-SPS antibodyAnti-SPS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89732/358210/STJ11100796_1__05587.1713122533.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89731/358209/STJ11100795_1__53467.1713122532.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
