• Western blot analysis of extracts of mouse testis, using SEMG2 antibody (STJ114744) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEMG2 antibody (383-582) (STJ114744)

SKU:
STJ114744

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEMG2 (383-582) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMG2
Gene ID: 6407
Uniprot ID: SEMG2_HUMAN
Immunogen Region: 383-582
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 383-582 of human SEMG2 (NP_002999.1).
Immunogen Sequence: HGKSQNQVRIPSQAQEYGHK ENKISYQSSSTEERRLNSGE KDVQKGVSKGSISIQTEEKI HGKSQNQVTIPSQDQEHGHK ENKMSYQSSSTEERRLNYGG KSTQKDVSQSSISFQIEKLV EGKSQIQTPNPNQDQWSGQN AKGKSGQSADSKQDLLSHEQ KGRYKQESSESHNIVITEHE VAQDDHLTQQYNEDRNPIST
Tissue Specificity Seminal vesicles, and to a much lesser extent, epididymis.
Post Translational Modifications Semenogelin-2 is thought to form both the 71 kDa polypeptide and, in its glycosylated form, the 76 kDa polypeptide.
Function Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
Protein Name Semenogelin-2
Semenogelin Ii
Sgii
Cellular Localisation Secreted
Alternative Antibody Names Anti-Semenogelin-2 antibody
Anti-Semenogelin Ii antibody
Anti-Sgii antibody
Anti-SEMG2 antibody

Information sourced from Uniprot.org

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