• Western blot analysis of extracts of various cell lines, using SEMA3F antibody (STJ113312) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SEMA3F antibody (686-785) (STJ113312)

SKU:
STJ113312

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEMA3F (686-785) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMA3F
Gene ID: 6405
Uniprot ID: SEM3F_HUMAN
Immunogen Region: 686-785
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 686-785 of human SEMA3F (NP_004177.3).
Immunogen Sequence: KHVVTRVQLHVLGRDAVHAA LFPPLSMSAPPPPGAGPPTP PYQELAQLLAQPEVGLIHQY CQGYWRHVPPSPREAPGAPR SPEPQDQKKPRNRRHHPPDT
Tissue Specificity Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
Function May play a role in cell motility and cell adhesion.
Protein Name Semaphorin-3f
Sema Iii/F
Semaphorin Iv
Sema Iv
Cellular Localisation Secreted
Alternative Antibody Names Anti-Semaphorin-3f antibody
Anti-Sema Iii/F antibody
Anti-Semaphorin Iv antibody
Anti-Sema Iv antibody
Anti-SEMA3F antibody

Information sourced from Uniprot.org

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