• Western blot analysis of extracts of various cell lines, using SDHB Rabbit monoclonal antibody (STJ11101459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SDHB antibody (181-280) [S9MR] (STJ11101459)

SKU:
STJ11101459

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SDHB (181-280) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHB
Gene ID: 6390
Uniprot ID: SDHB_HUMAN
Immunogen Region: 181-280
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 181-280 of human SDHB (P21912).
Immunogen Sequence: DGLYECILCACCSTSCPSYW WNGDKYLGPAVLMQAYRWMI DSRDDFTEERLAKLQDPFSL YRCHTIMNCTRTCPKGLNPG KAIAEIKKMMATYKEKKASV
Function Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Protein Name Succinate Dehydrogenase Ubiquinone Iron-Sulfur Subunit - Mitochondrial
Iron-Sulfur Subunit Of Complex Ii
Ip
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Succinate Dehydrogenase Ubiquinone Iron-Sulfur Subunit - Mitochondrial antibody
Anti-Iron-Sulfur Subunit Of Complex Ii antibody
Anti-Ip antibody
Anti-SDHB antibody
Anti-SDH antibody
Anti-SDH1 antibody

Information sourced from Uniprot.org

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