• Western blot analysis of extracts of U-87MG cells, using SATB2 antibody (STJ11103518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded rat brain using SATB2 rabbit monoclonal antibody (STJ11103518) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using SATB2 rabbit monoclonal antibody (STJ11103518) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse brain using SATB2 rabbit monoclonal antibody (STJ11103518) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SATB2 antibody (634-733) [S8MR] (STJ11103518)

SKU:
STJ11103518

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SATB2 (634-733) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SATB2
Gene ID: 23314
Uniprot ID: SATB2_HUMAN
Immunogen Region: 634-733
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 634-733 of human SATB2 (Q9UPW6).
Immunogen Sequence: HDVGLYPDQEAIHTLSAQLD LPKHTIIKFFQNQRYHVKHH GKLKEHLGSAVDVAEYKDEE LLTESEENDSEEGSEEMYKV EAEEENADKSKAAPAEIDQR
Tissue Specificity High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
Post Translational Modifications Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
Function Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
Protein Name Dna-Binding Protein Satb2
Special At-Rich Sequence-Binding Protein 2
Database Links Reactome: R-HSA-4551638
Reactome: R-HSA-8940973
Cellular Localisation Nucleus Matrix
Alternative Antibody Names Anti-Dna-Binding Protein Satb2 antibody
Anti-Special At-Rich Sequence-Binding Protein 2 antibody
Anti-SATB2 antibody
Anti-KIAA1034 antibody

Information sourced from Uniprot.org

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