• Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (STJ11101210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.

Anti-SARS-COV-2 N Protein-antibody (STJ11101210)


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Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: SARS-CoV-2
Short Description: Rabbit monoclonal antibody anti-SARS-COV-2 N Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000ELISA 1:1000-1:5000FCM 1:50-1:200
IHC 1:50-1:200
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at 4°C. Avoid freeze/thaw cycles.
Immunogen: Recombinant fusion protein of SARS-COV-2 Nucleoprotein.
Background Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Information sourced from Uniprot.org

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