• Western blot analysis of extracts of various cell lines, using SAR1A antibody (STJ29612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SAR1A antibody (1-198) (STJ29612)

SKU:
STJ29612

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SAR1A (1-198) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAR1A
Gene ID: 56681
Uniprot ID: SAR1A_HUMAN
Immunogen Region: 1-198
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SAR1A (NP_064535.1).
Immunogen Sequence: MSFIFEWIYNGFSSVLQFLG LYKKSGKLVFLGLDNAGKTT LLHMLKDDRLGQHVPTLHPT SEELTIAGMTFTTFDLGGHE QARRVWKNYLPAINGIVFLV DCADHSRLVESKVELNALMT DETISNVPILILGNKIDRTD AISEEKLREIFGLYGQTTGK GNVTLKELNARPMEVFMCSV LKRQGYGEGFRWLSQYID
Function Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Protein Name Gtp-Binding Protein Sar1a
Copii-Associated Small Gtpase
Cellular Localisation Endoplasmic Reticulum
Golgi Apparatus
Alternative Antibody Names Anti-Gtp-Binding Protein Sar1a antibody
Anti-Copii-Associated Small Gtpase antibody
Anti-SAR1A antibody
Anti-SAR1 antibody
Anti-SARA antibody
Anti-SARA1 antibody

Information sourced from Uniprot.org

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