• Western blot analysis of extracts of various cell lines, using S100A3 antibody (STJ25430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-S100A3 antibody (1-101) (STJ25430)

SKU:
STJ25430

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100A3 (1-101) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A3
Gene ID: 6274
Uniprot ID: S10A3_HUMAN
Immunogen Region: 1-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A3 (NP_002951.1).
Immunogen Sequence: MARPLEQAVAAIVCTFQEYA GRCGDKYKLCQAELKELLQK ELATWTPTEFRECDYNKFMS VLDTNKDCEVDFVEYVRSLA CLCLYCHEYFKDCPSEPPCS Q
Tissue Specificity Skin specific, specifically expressed at the inner endocuticle of hair fibers.
Post Translational Modifications More than half of the arginine residues undergo citrullination by PAD1 and PAD2. Arg-51 is specifically citrullinated by PAD3 and promotes tetramerization.
Function Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.
Protein Name Protein S100-A3
Protein S-100e
S100 Calcium-Binding Protein A3
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein S100-A3 antibody
Anti-Protein S-100e antibody
Anti-S100 Calcium-Binding Protein A3 antibody
Anti-S100A3 antibody
Anti-S100E antibody

Information sourced from Uniprot.org

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