• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using S100A12 Rabbit polyclonal antibody (STJ114373) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-S100A12 antibody (1-92) (STJ114373)

SKU:
STJ114373

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Host: Rabbit
Applications: IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100A12 (1-92) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A12
Gene ID: 6283
Uniprot ID: S10AC_HUMAN
Immunogen Region: 1-92
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A12 (NP_005612.1).
Immunogen Sequence: MTKLEEHLEGIVNIFHQYSV RKGHFDTLSKGELKQLLTKE LANTIKNIKDKAVIDEIFQG LDANQDEQVDFQEFISLVAI ALKAAHYHTHKE
Tissue Specificity Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various inflammatory disorders, such as rheumatoid arthritis, psoriatic arthritis, Crohn's disease, ulcerative colitis, and Kawasaki disease.
Function S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases.MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.
Protein Name Protein S100-A12
Cgrp
Calcium-Binding Protein In Amniotic Fluid 1
Caaf1
Calgranulin-C
Cagc
Extracellular Newly Identified Rage-Binding Protein
En-Rage
Migration Inhibitory Factor-Related Protein 6
Mrp-6
P6
Neutrophil S100 Protein
S100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin
Database Links Reactome: R-HSA-445989
Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-933542
Cellular Localisation Secreted
Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Predominantly Localized In The Cytoplasm
Upon Elevation Of The Intracellular Calcium Level
Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane
Upon Neutrophil Activation Is Secreted Via A Microtubule-Mediated
Alternative Pathway
Alternative Antibody Names Anti-Protein S100-A12 antibody
Anti-Cgrp antibody
Anti-Calcium-Binding Protein In Amniotic Fluid 1 antibody
Anti-Caaf1 antibody
Anti-Calgranulin-C antibody
Anti-Cagc antibody
Anti-Extracellular Newly Identified Rage-Binding Protein antibody
Anti-En-Rage antibody
Anti-Migration Inhibitory Factor-Related Protein 6 antibody
Anti-Mrp-6 antibody
Anti-P6 antibody
Anti-Neutrophil S100 Protein antibody
Anti-S100 Calcium-Binding Protein A12 Cleaved Into - Calcitermin antibody
Anti-S100A12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance