• Western blot analysis of extracts of various cell lines, using Reptin/RUVBL2 Rabbit monoclonal antibody (STJ11102022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Reptin/RUVBL2 Rabbit monoclonal antibody (STJ11102022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RUVBL2 antibody (350-450) [S2MR] (STJ11102022)

SKU:
STJ11102022

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Reptin/RUVBL2 (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUVBL2
Gene ID: 10856
Uniprot ID: RUVB2_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Reptin/RUVBL2 (Q9Y230).
Immunogen Sequence: LLDRLLIVSTTPYSEKDTKQ ILRIRCEEEDVEMSEDAYTV LTRIGLETSLRYAIQLITAA SLVCRKRKGTEVQVDDIKRV YSLFLDESRSTQYMKEYQDA F
Tissue Specificity Ubiquitously expressed. Highly expressed in testis and thymus.
Function Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity.hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome -DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. May play a role in regulating the composition of the U5 snRNP complex.
Protein Name Ruvb-Like 2
48 Kda Tata Box-Binding Protein-Interacting Protein
48 Kda Tbp-Interacting Protein
51 Kda Erythrocyte Cytosolic Protein
Ecp-51
Ino80 Complex Subunit J
Repressing Pontin 52
Reptin 52
Tip49b
Tip60-Associated Protein 54-Beta
Tap54-Beta
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-3214847
Cellular Localisation Nucleus Matrix
Nucleus
Nucleoplasm
Cytoplasm
Membrane
Dynein Axonemal Particle
Mainly Localized In The Nucleus
Associated With Nuclear Matrix Or In The Nuclear Cytosol
Although It Is Also Present In The Cytoplasm And Associated With The Cell Membranes
Alternative Antibody Names Anti-Ruvb-Like 2 antibody
Anti-48 Kda Tata Box-Binding Protein-Interacting Protein antibody
Anti-48 Kda Tbp-Interacting Protein antibody
Anti-51 Kda Erythrocyte Cytosolic Protein antibody
Anti-Ecp-51 antibody
Anti-Ino80 Complex Subunit J antibody
Anti-Repressing Pontin 52 antibody
Anti-Reptin 52 antibody
Anti-Tip49b antibody
Anti-Tip60-Associated Protein 54-Beta antibody
Anti-Tap54-Beta antibody
Anti-RUVBL2 antibody
Anti-INO80J antibody
Anti-TIP48 antibody
Anti-TIP49B antibody
Anti-CGI-46 antibody

Information sourced from Uniprot.org

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