• Western blot analysis of various lysates using RSPH14 Rabbit polyclonal antibody (STJ117625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RSPH14 antibody (1-348) (STJ117625)

SKU:
STJ117625

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSPH14
Gene ID: 27156
Uniprot ID: RSP14_HUMAN
Immunogen Region: 1-348
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human RSPH14 (NP_055248.1).
Immunogen Sequence: MAHSQNSLELPININATQIT TAYGHRALPKLKEELQSEDL QTRQKALMALCDLMHDPECI YKAMNIGCMENLKALLKDSN SMVRIKTTEVLHITASHSVG RYAFLEHDIVLALSFLLNDP SPVCRGNLYKAYMQLVQVPR GAQEIISKGLISSLVWKLQV EVEEEEFQEFILDTLVLCLQ EDATEALGSNVVLVLKQKLL SANQNIRSKAARALLNVSIS REGKKQVCHFDVIPILVHL
Tissue Specificity Expressed in adult cerebellum, spinal cord, spleen, skeletal muscle and some/5 out of 9 rhabdoid tumors. Detected in fetal brain, lung, liver and kidney.
Function Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia.
Protein Name Radial Spoke Head 14 Homolog
Rhabdoid Tumor Deletion Region Protein 1
Cellular Localisation Cytoplasm
Cytoskeleton
Flagellum Axoneme
Alternative Antibody Names Anti-Radial Spoke Head 14 Homolog antibody
Anti-Rhabdoid Tumor Deletion Region Protein 1 antibody
Anti-RSPH14 antibody
Anti-RTDR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance