• Western blot analysis of extracts of various cell lines, using RRM1 antibody (STJ115612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Immunofluorescence analysis of HeLa cells using RRM1 rabbit polyclonal antibody (STJ115612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RRM1 antibody (543-792) (STJ115612)

SKU:
STJ115612

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRM1 (543-792) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRM1
Gene ID: 6240
Uniprot ID: RIR1_HUMAN
Immunogen Region: 543-792
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 543-792 of human RRM1 (NP_001024.1).
Immunogen Sequence: SCDLAKEQGPYETYEGSPVS KGILQYDMWNVTPTDLWDWK VLKEKIAKYGIRNSLLIAPM PTASTAQILGNNESIEPYTS NIYTRRVLSGEFQIVNPHLL KDLTERGLWHEEMKNQIIAC NGSIQSIPEIPDDLKQLYKT VWEISQKTVLKMAAERGAFI DQSQSLNIHIAEPNYGKLTS MHFYGWKQGLKTGMYYLRTR PAANPIQFTLNKEKLKDKEK VSKEEEEKERNTAAMVCSL
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Protein Name Ribonucleoside-Diphosphate Reductase Large Subunit
Ribonucleoside-Diphosphate Reductase Subunit M1
Ribonucleotide Reductase Large Subunit
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ribonucleoside-Diphosphate Reductase Large Subunit antibody
Anti-Ribonucleoside-Diphosphate Reductase Subunit M1 antibody
Anti-Ribonucleotide Reductase Large Subunit antibody
Anti-RRM1 antibody
Anti-RR1 antibody

Information sourced from Uniprot.org

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