| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RPGR |
| Gene ID: |
6103 |
| Uniprot ID: |
RPGR_HUMAN |
| Immunogen Region: |
1-260 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RPGR (NP_001030025.1). |
| Immunogen Sequence: |
MREPEELMPDSGAVFTFGKS KFAENNPGKFWFKNDVPVHL SCGDEHSAVVTGNNKLYMFG SNNWGQLGLGSKSAISKPTC VKALKPEKVKLAACGRNHTL VSTEGGNVYATGGNNEGQLG LGDTEERNTFHVISFFTSEH KIKQLSAGSNTSAALTEDGR LFMWGDNSEGQIGLKNVSNV CVPQQVTIGKPVSWISCGYY HSAFVTTDGELYVFGEPENG KLGLPNQLLGNHRTPQLVS |
| Tissue Specificity | Heart, brain, placenta, lung, liver, muscle, kidney, retina, pancreas and fetal retinal pigment epithelium. Isoform 3 is found only in the retina. Colocalizes with RPGRIP1 in the outer segment of rod photoreceptors and cone outer segments. |
| Post Translational Modifications | Prenylated. |
| Function | Could be a guanine-nucleotide releasing factor. Plays a role in ciliogenesis. Probably regulates cilia formation by regulating actin stress filaments and cell contractility. Plays an important role in photoreceptor integrity. May play a critical role in spermatogenesis and in intraflagellar transport processes. May be involved in microtubule organization and regulation of transport in primary cilia. |
| Protein Name | X-Linked Retinitis Pigmentosa Gtpase Regulator |
| Cellular Localisation | CytoplasmCytoskeletonFlagellum AxonemeGolgi ApparatusCell ProjectionCiliumIn The Retinal Photoreceptor Cell LayerLocalizes At The Connecting CiliumColocalizes With Whrn In The Photoreceptor Connecting CiliumColocalizes With Cep290 In The Photoreceptor Connecting CiliumColocalizes With Rpgrip1 In The Photoreceptor Connecting CiliumIsoform 6: CytoplasmMicrotubule Organizing CenterCentrosomeCilium Basal BodyCilium Axoneme |
| Alternative Antibody Names | Anti-X-Linked Retinitis Pigmentosa Gtpase Regulator antibodyAnti-RPGR antibodyAnti-RP3 antibodyAnti-XLRP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")