• Western blot analysis of extracts of various cell lines, using ROBO2 antibody (STJ112110) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-ROBO2 antibody (450-660) (STJ112110)

SKU:
STJ112110

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ROBO2 (450-660) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ROBO2
Gene ID: 6092
Uniprot ID: ROBO2_HUMAN
Immunogen Region: 450-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ROBO2 (NP_001122401.1).
Immunogen Sequence: TALLKCKATGDPLPVISWLK EGFTFPGRDPRATIQEQGTL QIKNLRISDTGTYTCVATSS SGETSWSAVLDVTESGATIS KNYDLSDLPGPPSKPQVTDV TKNSVTLSWQPGTPGTLPAS AYIIEAFSQSVSNSWQTVAN HVKTTLYTVRGLRPNTIYLF MVRAINPQGLSDPSPMSDPV RTQDISPPAQGVDHRQVQKE LGDVLVRLHNP
Function Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
Protein Name Roundabout Homolog 2
Database Links Reactome: R-HSA-376176
Reactome: R-HSA-428542
Reactome: R-HSA-8985801
Reactome: R-HSA-9010553
Reactome: R-HSA-9010642
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Roundabout Homolog 2 antibody
Anti-ROBO2 antibody
Anti-KIAA1568 antibody

Information sourced from Uniprot.org

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